Question

In respect to PCR technique, in a degraded biological sample, the longer the amplicon length:

• A. The higher the risk of failure in PCR
• B. The lowest the risk of failure in PCR
• C. The higher the risk of disturbing coding region
• D. The higher temperature is required to get better results

Hint:

When working with degraded samples, design primers for shorter amplicons to improve the likelihood of successful PCR amplification.

Answer 1

The Correct Option is A

Degraded DNA samples often contain fragmented DNA strands. PCR amplification relies on intact template strands, and the longer the amplicon length, the higher the probability of template failure. For degraded DNA, short amplicon targets (e.g., miniSTRs) are often used to ensure successful amplification despite template degradation.