Question:

In plant biotechnology, PEG is used in

Updated On: Jun 18, 2022
  • protoplast isolation
  • cell culture preparation
  • protoplast fusion
  • hardening
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The Correct Option is C

Solution and Explanation

Somatic hybridization involves the fusion of protoplasts of two different species which resulted in hybrid. Naked protoplasts are obtained by dissolution of their cell walls by the mecerating enzymes such as pectinase and cellulase. Fusion of protoplasts from two different varieties can be enhanced by treating with polyethylene glycol (PEG) in presence of high voltage electric current.
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Concepts Used:

Biotechnology

Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."

Principles of Biotechnology:

Among many, the two core techniques that entitled the birth of modern biotechnology are :

  1. Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
  2. Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.