Question:

In Hershey and Chase experiments, radioactive $^{32}P$ was used to culture bacteriophages which resulted in radioactive:

Updated On: Jun 6, 2022
  • Viral $DNA$
  • Bacterial capsule
  • Viral proteins
  • Plasma membrane of bacteria
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Solution and Explanation

The unequivocal proof that DNA is the genetic material came from the experiments of Alfred Hershey and Martha Chase (1952). They worked with viruses that infect bacteria called bacteriophages. They grew some viruses
on a medium that contained radioactive phosphorus ($^{32}P$) and some others on medium that contained radioactive sulfur ($^{35}S$). Viruses grown in the presence of radioactive phosphorus contained radioactive DNA but not radioactive protein because DNA contains phosphorus but protein does not. Similarly, viruses grown on radioactive sulfur contained radioactive protein but not radioactive DNA because DNA does not contain sulfur.
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Concepts Used:

The Molecular Basis of Inheritance

DNA Replication:

DNA synthesis is commenced at particular points within the DNA strand referred to as ‘origins’, which are certain coding regions. There are numerous origin sites, and when replication of DNA starts, these sites are mentioned as replication forks. Within the replication, the complex is the enzyme DNA Helicase, so that they can be utilized as a template for replication. DNA Primase is another enzyme that's essential in DNA replication.

RNA:

Ribonucleic acid (RNA) is an essential biological macromolecule that exists all together in biological cells. It is principally involved in the synthesis of proteins, that carry the messenger instructions from DNA, which itself contains the genetic instructions needed for the event and maintenance of life. In some viruses, RNA, in spite of DNA, carries genetic information.

Genetic Code:

Genetic code is the term we use in the manner that the four bases of DNA--the A, C, G, and Ts--are strung together in a way that the ribosome, the cellular machinery, can read them and switch them into a protein. In the ordering, every three nucleotides during a row count as a triplet and code for one amino alkanoic acid.

Read More: Molecular Basis of Inheritance