Question

In Gambir-fluorescin test the petroleum spirit layer shows

• A. Green fluorescence
• B. Blue fluorescence
• C. Yellow fluorescence
• D. Red fluorescence

Answer 1

The Correct Option is A

The Gambir-fluorescin test involves the formation of a colored fluorescent complex under specific conditions. This test is used in the field of pharmaceutical analysis to identify certain compounds based on their fluorescent properties.

In this specific test with Gambir and fluorescein, the petroleum spirit layer is observed for its fluorescence color. Fluorescence occurs when a substance absorbs light at one wavelength and emits light at another, typically longer wavelength. 

The question asks for the color of fluorescence observed in the petroleum spirit layer during the test. The options provided are:

1. Green fluorescence
2. Blue fluorescence
3. Yellow fluorescence
4. Red fluorescence

According to the principles of the Gambir-fluorescin test, the petroleum spirit layer usually exhibits green fluorescence.

This is because fluorescein, when dissolved in a suitable organic solvent like petroleum spirit, typically shows strong green fluorescence under UV light. The fluorescein is excited by ultraviolet or blue light, leading it to emit green light due to its molecular structure and electronic properties.

Hence, the correct answer is:

Green fluorescence