Step 1: Given data.
- Protein content = 20% of the cell weight.
- Density of cell = 1.2 g/mL.
- Volume of cell = \( 4 \times 10^{-9} \) mL.
Step 2: Calculate the mass of the cell.
Mass of the cell = Density × Volume = \( 1.2 \, \text{g/mL} \times 4 \times 10^{-9} \, \text{mL} = 4.8 \times 10^{-9} \, \text{g} \).
Step 3: Calculate the mass of protein.
Mass of protein = 20% of the mass of the cell = \( 0.2 \times 4.8 \times 10^{-9} \, \text{g} = 9.6 \times 10^{-10} \, \text{g} \).
Step 4: Convert mass of protein to mg.
Mass of protein in mg = \( 9.6 \times 10^{-10} \, \text{g} = 9.6 \times 10^{-7} \, \text{mg} \).
Step 5: Calculate the concentration of protein.
Concentration = \( \frac{\text{Mass of protein}}{\text{Volume of cell}} = \frac{9.6 \times 10^{-7} \, \text{mg}}{4 \times 10^{-9} \, \text{mL}} = 240 \, \text{mg/mL} \).
Step 6: Conclusion.
The correct answer is (D) 240.
| List I | List II |
|---|---|
| A. Adenosine | III. Nucleoside |
| B. Adenylic acid | II. Nucleotide |
| C. Adenine | I. Nitrogen base |
| D. Alanine | IV. Amino acid |
Match the LIST I (Enzyme) with LIST II (Catabolic Products)
| LIST-I | LIST-II | ||
|---|---|---|---|
| (Enzyme) | (Catabolic Products) | ||
| A | \(\beta\)-galactosidase | III | Galactose + glucose |
| B | Lecithinase | I | Choline + H$_3$PO$_4$ + fat |
| C | Urease | IV | CO$_2$ + NH$_3$ |
| D | Lipase | II | Glycerol + fatty acids |
Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 