An air filled parallel plate electrostatic actuator is shown in the figure. The area of each capacitor plate is $100 \mu m \times 100 \mu m$. The distance between the plates $d_0 = 1 \mu m$ when both the capacitor charge and spring restoring force are zero as shown in Figure (a). A linear spring of constant $k = 0.01 N/m$ is connected to the movable plate. When charge is supplied to the capacitor using a current source, the top plate moves as shown in Figure (b). The magnitude of minimum charge (Q) required to momentarily close the gap between the plates is ________ $\times 10^{-14} C$ (rounded off to two decimal places). Note: Assume a full range of motion is possible for the top plate and there is no fringe capacitance. The permittivity of free space is $\epsilon_0 = 8.85 \times 10^{-12} F/m$ and relative permittivity of air ($\epsilon_r$) is 1.
Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 