- Without a selectable marker, it becomes difficult to distinguish between recombinant and non-recombinant cells.
- Additional screening methods, such as PCR or DNA sequencing, are needed, which increases time and cost.
List I | List II | ||
|---|---|---|---|
| A | Mesozoic Era | I | Lower invertebrates |
| B | Proterozoic Era | II | Fish & Amphibia |
| C | Cenozoic Era | III | Birds & Reptiles |
| D | Paleozoic Era | IV | Mammals |