- Without a selectable marker, it becomes difficult to distinguish between recombinant and non-recombinant cells.
- Additional screening methods, such as PCR or DNA sequencing, are needed, which increases time and cost.
List I | List II | ||
---|---|---|---|
A | Mesozoic Era | I | Lower invertebrates |
B | Proterozoic Era | II | Fish & Amphibia |
C | Cenozoic Era | III | Birds & Reptiles |
D | Paleozoic Era | IV | Mammals |
A school is organizing a debate competition with participants as speakers and judges. $ S = \{S_1, S_2, S_3, S_4\} $ where $ S = \{S_1, S_2, S_3, S_4\} $ represents the set of speakers. The judges are represented by the set: $ J = \{J_1, J_2, J_3\} $ where $ J = \{J_1, J_2, J_3\} $ represents the set of judges. Each speaker can be assigned only one judge. Let $ R $ be a relation from set $ S $ to $ J $ defined as: $ R = \{(x, y) : \text{speaker } x \text{ is judged by judge } y, x \in S, y \in J\} $.