- Without a selectable marker, it becomes difficult to distinguish between recombinant and non-recombinant cells.
- Additional screening methods, such as PCR or DNA sequencing, are needed, which increases time and cost.
List I | List II | ||
|---|---|---|---|
| A | Mesozoic Era | I | Lower invertebrates |
| B | Proterozoic Era | II | Fish & Amphibia |
| C | Cenozoic Era | III | Birds & Reptiles |
| D | Paleozoic Era | IV | Mammals |
A battery of emf \( E \) and internal resistance \( r \) is connected to a rheostat. When a current of 2A is drawn from the battery, the potential difference across the rheostat is 5V. The potential difference becomes 4V when a current of 4A is drawn from the battery. Calculate the value of \( E \) and \( r \).
If \(\begin{vmatrix} 2x & 3 \\ x & -8 \\ \end{vmatrix} = 0\), then the value of \(x\) is: