Question

(iii) A cloning vector does not have a selectable marker. How will it affect the process of cloning?

Hint:

Selectable markers simplify the identification of recombinant DNA in host cells.

Answer 1

Selectable markers are essential for identifying recombinant cells. Without them: 1. Increased complexity: Alternative methods like PCR or sequencing would be needed, increasing cost and time. 2. Higher error rate: Non-recombinant cells may outgrow recombinant ones, complicating the process. 3. Importance of markers: Antibiotic resistance genes, for example, enable easy identification by allowing only transformed cells to grow. Their absence significantly complicates the cloning workflow and reduces efficiency. % Quick tip