The pBR322 is a commonly used E. coli cloning vector that contains several restriction enzyme sites for cloning purposes. To identify the missing restriction sites A, B, and C, we must refer to the typical arrangement of restriction sites present in the pBR322 vector.
In pBR322, the vector contains multiple restriction sites, and BamHI, ClaI, and PstI are among the commonly used restriction enzymes.
A - BamHI: BamHI is a frequently used restriction enzyme that cuts at a specific sequence.
B - ClaI: ClaI is another restriction enzyme used for cutting at a specific site within the vector.
C - PstI: PstI is also commonly found in cloning vectors like pBR322, used for cutting at specific sequences.
Thus, the missing restriction sites are:
A - BamHI
B - ClaI
C - PstI
The correct answer is (1) A - BamHI; B - ClaI; C - PstI.
Was this answer helpful?
0
0
Hide Solution
Verified By Collegedunia
Approach Solution -2
In the pBR322 vector, the correct restriction sites are: A - BamHI, B - ClaI, and C - PstI. These sites are strategically placed to allow easy insertion and modification within the antibiotic resistance regions of pBR322.
