To determine the missing restriction sites in the E. coli cloning vector pBR322, we need to understand the typical layout and restriction sites present in this commonly used vector. pBR322 contains multiple cloning sites for efficient insertion of foreign DNA.
Key Restriction Sites in pBR322:
BamHI: Typically located in the tetracycline resistance gene region.
ClaI: Commonly found near the replication origin.
PstI: Generally positioned in the ampicillin resistance gene region.
Step-by-step Identification:
Assess position A: Based on the vector map, the site must be a restriction site that influences tetracycline resistance. In pBR322, BamHI is known to be in this area.
Assess position B: Located close to the replication origin, which is a common spot for ClaI.
Assess position C: Situated within the ampicillin resistance region where PstI is usually found.
Thus, correlating the standard locations of these restriction enzymes within pBR322 verifies that the correct sequence of restriction sites is A - BamHI; B - ClaI; C - PstI.
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The pBR322 is a commonly used E. coli cloning vector that contains several restriction enzyme sites for cloning purposes. To identify the missing restriction sites A, B, and C, we must refer to the typical arrangement of restriction sites present in the pBR322 vector.
In pBR322, the vector contains multiple restriction sites, and BamHI, ClaI, and PstI are among the commonly used restriction enzymes.
A - BamHI: BamHI is a frequently used restriction enzyme that cuts at a specific sequence.
B - ClaI: ClaI is another restriction enzyme used for cutting at a specific site within the vector.
C - PstI: PstI is also commonly found in cloning vectors like pBR322, used for cutting at specific sequences.
Thus, the missing restriction sites are:
A - BamHI
B - ClaI
C - PstI
The correct answer is (1) A - BamHI; B - ClaI; C - PstI.
