Question

Identify the incorrect statement regarding PCR.

• A. Two sets of primers are required during polymerisation.
• B. The process of replication is repeated multiple times to produce one billion copies.
• C. Thermostable DNA polymerase is used for extension of primers.
• D. Annealing is required to separate both the strands of template DNA.

Hint:

Remember — Denaturation separates DNA strands, Annealing binds primers, and Extension synthesizes new DNA strands.

Answer 1

The Correct Option is D

In PCR, denaturation is the step where the double-stranded DNA is heated to separate into two strands. Annealing refers to the cooling phase where primers bind to their complementary sequences on the template strands. Hence, option (D) is incorrect because separation happens during denaturation, not annealing.