Humans possess a total of 23 pairs of chromosomes in their cells. Out of these, 22 pairs are autosomal chromosomes, which are non-sex chromosomes and carry the bulk of genetic information ranging from hair color to metabolic enzymes. The autosomal chromosomes are numbered from 1 to 22 based on their size, with chromosome 1 being the largest and chromosome 22 being relatively smaller. The 23rd pair consists of the sex chromosomes, X and Y, which determine an individual's sex. Thus, in normal humans, there are 22 pairs of autosomal chromosomes.
Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 