In bacterial translation elongation, tRNA translocation is a crucial step where the tRNA moves from the P (peptidyl) site to the E (exit) site of the ribosome. During this process, an elongation factor known as EF-G is required, which facilitates the movement of the ribosome along the mRNA. This translocation is powered by the hydrolysis of a GTP molecule. Therefore, each translocation event consumes exactly one GTP molecule.

Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 