Question

High probability of target injuries for following technique is used for DNA transfer

• A. Gene Gun
• B. PCR
• C. RT PCR
• D. Electrophoresis

Hint:

• Gene Gun Physical DNA transfer, can cause cell/tissue damage.
• PCR/RT PCR DNA amplification techniques, no physical transfer.
• Electrophoresis DNA separation technique, no transfer involved.

Answer 1

The Correct Option is A

The technique with a high probability of target injuries during DNA transfer is Gene Gun. The Gene Gun method, also known as biolistic particle delivery system, involves the use of high-speed particle bombardment to deliver foreign DNA into cells. This technique can cause physical damage to target cells due to the penetration of microprojectiles. The DNA-coated particles are propelled into cells at high velocities, potentially leading to cellular injury or even cell death.

In comparison, the other techniques mentioned do not involve physical penetration of cells:

• PCR (Polymerase Chain Reaction) is a method to amplify DNA segments and does not involve DNA transfer into cells.
• RT PCR (Reverse Transcription PCR) is used for detecting RNA expression and involves converting RNA into DNA, also without physical DNA transfer into cells.
• Electrophoresis is a method to separate DNA fragments based on size using an electric field and does not involve DNA transfer into cells.