Question

For isolation of DNA a fungus is treated with which enzyme?

• A. Lysozyme
• B. Cellulase
• C. DNase
• D. Chitinase

Hint:

Always match enzyme with cell wall component: Lysozyme → bacteria, Cellulase → plants, Chitinase → fungi.

Answer 1

The Correct Option is D

Step 1: Cell wall composition.
Fungal cell walls are mainly composed of chitin. To break open fungal cells for DNA isolation, the enzyme chitinase is used.
Step 2: Analyzing options.
(A) Lysozyme: Correct for bacteria (acts on peptidoglycan).
(B) Cellulase: Correct for plant cells (acts on cellulose).
(C) DNase: Incorrect — It degrades DNA, not used for extraction.
(D) Chitinase: Correct — Breaks down fungal cell walls.
Step 3: Conclusion.
Thus, Chitinase is used for DNA isolation from fungi.