Question

ELISA is based on the principle of:

• A. Antigen – Antigen interaction
• B. B-cells and T-cells interaction
• C. Antigen – Antibody interaction
• D. T-cell – Antibody interaction

Answer 1

The Correct Option is C

ELISA (Enzyme-Linked Immunosorbent Assay) is a biochemical technique used mainly in immunology to detect the presence of an antibody or antigen in a sample. This technique is based on the specific binding between an antigen and an antibody, which allows for highly specific detection of substances. In ELISA, enzymes linked to antibodies produce a detectable signal, usually colorimetric, which indicates the presence of the target molecule.

Answer 2

ELISA (Enzyme-Linked Immunosorbent Assay) is a widely used laboratory technique that is based on the interaction between antigens and antibodies. It is used for detecting and quantifying substances such as proteins, hormones, or antibodies in a sample. The principle behind ELISA involves the specific binding of an antibody to its corresponding antigen, which allows the detection of the presence of the antigen in a sample when a color change or signal is produced, typically through an enzyme-substrate reaction.