Question:

E.coli having $pBR322$ with $DNA$ insert at BamHI site cannot grow in medium containing tetracycline. Recognition site for BamHI is present in $tet^R$ region of $pBR322$.

Updated On: Jul 6, 2022
  • If both assertion and reason are true and reason is the correct explanation of assertion
  • If both assertion and reason are true but reason is not the correct explanation of assertion
  • If assertion is true but reason is false
  • If both assertion and reason are false
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The Correct Option is A

Solution and Explanation

$pBR322$ has recognition sites for several commonly used restriction enzymes. Recognition site for BamHI is present in tetr region i.e., region responsible for tetracycline resistance. When an insert is added at the BamHI recognition site the gene for tetracycline resistance becomes non-functional and the recombinant bacteria with plasmid $pBR322$ that has $DNA$ insert at BamHI lose tetracycline resistance.
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Notes on biotechnology

Concepts Used:

Biotechnology

Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."

Principles of Biotechnology:

Among many, the two core techniques that entitled the birth of modern biotechnology are :

  1. Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
  2. Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.