Question

Draw a schematic diagram of the cloning vector pBR 322 and label
(1) Bam HI site
(2) gene for ampicillin resistance
(3) ‘ori’
(4) ‘rop’ gene.

Hint:

The pBR 322 vector is a widely used cloning vector in genetic engineering due to its multiple cloning sites and selectable markers.

Answer 1

\begin{figure}[h!] \centering \includegraphics[width=0.6\textwidth]{Screenshot 2025-01-17 102347.png} \caption{Schematic representation of pBR322 cloning vector, highlighting important sites such as BamHI, EcoRI, origin of replication (ori), ampicillin resistance gene, and tetracycline resistance gene.} \label{fig:pbr322} \end{figure} A schematic diagram of pBR 322 must include: The origin of replication (‘ori’). Ampicillin resistance gene. Restriction site for Bam HI. ‘rop’ gene regulating plasmid copy number.