
To determine which regions of the photosynthetic filamentous alga will have maximum bacterial congregation, we need to understand the relationship between light wavelength, photosynthesis, and oxygen production.
The bacteria migrate towards high oxygen concentration areas, which are produced in regions where photosynthesis is most active. Photosynthesis in algae is influenced by the availability of light wavelengths that chlorophyll and other pigments absorb effectively.

The key points to note are:
Given these observations, the regions where photosynthesis, and therefore oxygen production, will be maximum are Regions P and R. Consequently, the regions with the maximum bacterial congregation will be Regions P and R.
Conclusion: The correct answer is P and R, where the bacterial congregation will be maximum due to higher oxygen production.

Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 