Question:

Cloning with lambda replacement vectors involves the following steps. Arrange them from first to last, in the order they are performed.
A. Packaging of DNA to form viable phage particles 
B. Using DNA ligase to connect lambda fragments to foreign DNA 
C. Isolating vector DNA from phage particles 
D. Infecting E-coli cells, with recombinant phage 
E. Cutting vector DNA with suitable restriction enzyme 
Choose the correct answer from the options given below:

Updated On: Mar 16, 2024
  • A, C, B, E, D
  • D, C, E, B, A
  • B, A, C, E, D
  • C, E, B, A, D
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The Correct Option is D

Solution and Explanation

The correct answer is (D) : C, E, B, A, D.
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