Question:

Chloroplasts are disrupted and the stroma separated from the lamella. The isolated stroma will fix CO$_2$, if it is supplied with:

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The Calvin cycle was elucidated using isolated chloroplast stroma, proving light-independent CO$_2$ fixation.
  • ATP and NADPH
  • Oxygen
  • Light
  • Carotenoid
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The Correct Option is A

Solution and Explanation


Calvin cycle requirements:
Stroma contains all enzymes for CO$_2$ fixation
Requires energy (ATP) and reducing power (NADPH)
Light not needed directly (dark reactions)
Oxygen inhibits RuBisCO activity
Carotenoids are light
harvesting pigments
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