In grapefruit, the bitterness often comes from a compound called limonin, which is formed after the extraction of juice under acidic conditions. This bitter compound belongs to a class of compounds known as limonoids, which are naturally found in citrus fruits, especially in the seeds and pulp. Limonin is particularly known for being bitter, and its concentration increases when the juice is exposed to acidic conditions, such as during extraction. It is responsible for the characteristic bitter taste found in grapefruit juice.
- Quinine (A) is a bitter alkaloid found in tonic water and is unrelated to grapefruit juice.
- Theobromine (B) is a compound found in cocoa and chocolate and has a mild bitter taste, but it is not associated with grapefruit.
- Isohumulone (C) is a bitter compound found in hops and is involved in beer brewing, not in grapefruit.
The bitterness that develops in grapefruit juice is primarily due to limonin (D), which is formed from limonoid compounds when the fruit juice is extracted under acidic conditions.
Thus, the correct answer is Limonin.
Final Answer: \boxed{(D) Limonin}