Question

Arrange the following steps involved in hybridization of rose flower crop in correct orders?
(A) Emasculation of flowers of female parents.
(B) Pollination
(C) Harvesting and storing of F\(_1\) seeds
(D) Bagging and tagging
(E) Choice and evaluation of pollen and female parents.
Choose the correct answer from the options given below:

• A. (C), (D), (E), (B), (A)
• B. (E), (A), (D), (B), (C)
• C. (A), (D), (E), (C), (B)
• D. (C), (B), (E), (A), (D)

Hint:

Standard steps in plant hybridization: 1. Select Parents (E): Choose male and female parents with desired traits. 2. Emasculate (A): Remove anthers from female parent's flower (if bisexual) before pollen sheds. 3. Bag & Tag (D): Protect emasculated flower from stray pollen and label it. 4. Pollinate (B): Apply pollen from male parent to stigma of female parent. Re-bag. 5. Harvest Seeds (C): Collect mature F\(_1\) hybrid seeds from the fruit.

Answer 1

The Correct Option is B

Hybridization is a controlled process of crossing two genetically different parent plants to produce offspring with desired traits. The steps involved are generally sequential:
(E) Choice and evaluation of pollen and female parents: The very first step is to select suitable parent plants. The female parent (seed parent) and male parent (pollen parent) are chosen based on the characteristics desired in the hybrid. Their flowering synchrony and health are also evaluated.
(A) Emasculation of flowers of female parents: If the chosen female parent has bisexual flowers (like most roses), its anthers (male parts) must be removed before they mature and shed pollen to prevent self-pollination. This is done when the flower bud is at the right stage, usually just before it opens.
(D) Bagging and tagging: After emasculation, the flower of the female parent is covered with a bag (e.g., paper or muslin) to prevent contamination by unwanted foreign pollen. The flower is also tagged with details of the parents and date of emasculation/pollination.
(B) Pollination: When the stigma of the emasculated flower becomes receptive, pollen collected from the chosen male parent is carefully applied to it. The flower is then re-bagged.
(C) Harvesting and storing of F\(_1\) seeds: After successful fertilization, the fruit (hip in roses) develops. Once the fruit is mature, it is harvested, and the F\(_1\) seeds (hybrid seeds) are extracted, cleaned, dried, and stored properly until they are ready for sowing to raise the F\(_1\) generation. So, the correct sequence is E \(\rightarrow\) A \(\rightarrow\) D \(\rightarrow\) B \(\rightarrow\) C. This corresponds to option (2). (E), (A), (D), (B), (C)