Let the amount of mixture released each time be \( x \) litres.
The total volume of the cylinder is 8 litres, and initially, the oxygen content is 16% of the total volume. Therefore, the initial volume of oxygen is:
\[
\text{Initial oxygen volume} = \frac{16}{100} \times 8 = 1.28 \text{ litres}
\]
When \( x \) litres of the mixture is released, the volume of oxygen is reduced, and an equal amount of nitrogen is added. After the first replacement, the volume of oxygen left is:
\[
\text{Oxygen volume after first replacement} = \left( 1 - \frac{x}{8} \right) \times 1.28
\]
Now, the same amount of the mixture is removed again. After the second replacement, the volume of oxygen is reduced further, and the volume of oxygen left becomes 9% of the total volume. Therefore:
\[
\text{Final oxygen volume} = \frac{9}{100} \times 8 = 0.72 \text{ litres}
\]
Using the formula for the oxygen volume after both replacements, we get:
\[
\left( 1 - \frac{x}{8} \right)^2 \times 1.28 = 0.72
\]
Solving this equation:
\[
\left( 1 - \frac{x}{8} \right)^2 = \frac{0.72}{1.28} = \frac{9}{16}
\]
\[
1 - \frac{x}{8} = \frac{3}{4}
\]
\[
\frac{x}{8} = \frac{1}{4}
\]
\[
x = 2 \text{ litres}
\]
Thus, the amount of mixture released each time is \( 2 \) litres.