Question:

All expression vectors are cloning vectors and vice versa. Expression vectors have at least the regulatory sequences i.e., promoters, operators, ribosomal binding sites, etc. having optimum function in the chosen control but not origin of replication.

Updated On: Jul 6, 2022
  • If both assertion and reason are true and reason is the correct explanation of assertion
  • If both assertion and reason are true but reason is not the correct explanation of assertion
  • If assertion is true but reason is false
  • If both assertion and reason are false
Hide Solution
collegedunia
Verified By Collegedunia

The Correct Option is D

Solution and Explanation

All vectors used for propagation of $DNA$ inserts in a suitable host are called cloning vectors. But when a vector is designed for the expression i.e., production of the protein specified by the $DNA$ insert, it is termed as expression vector, Expression vectors need regulatory machinery suitable for the host, e.g., if an eukaryotic gene is to be expressed in prokaryotes, the expression vectors should contain gene without introns because prokaryotes lack introns removal machinery for transcripts. But all vectors, including expression vectors must contain 'origin of replication' so that they can replicate when the host cell divides and their presence is ensured in all progeny.
Was this answer helpful?
0
0

Top Questions on Biotechnology

View More Questions

Concepts Used:

Biotechnology

Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."

Principles of Biotechnology:

Among many, the two core techniques that entitled the birth of modern biotechnology are :

  1. Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
  2. Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.