Question

A suicide plasmid vector lacks the following

• A. antibiotic marker
• B. origin of replication
• C. multiple cloning sites
• D. site for integration

Hint:

Suicide vectors are tools in genetic engineering used to force chromosomal integration by lacking a functional origin of replication in the host.

Answer 1

The Correct Option is B

Step 1: Understand what a suicide plasmid is. 
A suicide plasmid is a type of plasmid vector that cannot replicate in a target host cell due to the absence of a functional origin of replication recognized by the host. 
Step 2: Characteristics of a suicide vector.
It typically contains an antibiotic resistance marker to select for transformed cells.
It includes multiple cloning sites to insert foreign DNA.
It may have integration sites for recombination into the host genome.
However, it lacks a compatible origin of replication, which prevents it from replicating autonomously in the target host.
Step 3: Conclude the correct answer. 
Since the key characteristic of a suicide vector is its inability to replicate in the host due to absence of a suitable origin of replication, option (2) is correct.