Question:

A gene whose expression helps to identify transformed cell is known as :

Updated On: Aug 20, 2024
  • Vector
  • Plasmid
  • Structural gene
  • Selectable marker
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The Correct Option is D

Approach Solution - 1

In recombinant DNA technology, selectable markers helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.
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Approach Solution -2

In recombinant DNA technology, a selectable marker is a gene that allows scientists to identify cells that have successfully taken up foreign DNA (transformation).

  • These marker genes typically encode for antibiotic resistance. For instance, a plasmid might contain a gene for ampicillin resistance.
  • After introducing the plasmid containing the foreign DNA and the selectable marker into a culture of cells, scientists can add the antibiotic ampicillin to the culture medium.
  • Only the cells that have taken up the plasmid and the selectable marker gene will survive because they will be resistant to the antibiotic.

Thus, the right answer to this question is a selectable marker.

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Concepts Used:

Biotechnology

Biotechnology is a vast field of biology that involves the creation, production, and modification of useful products for human welfare using both the technology and the execution of living organisms and their components. An agricultural engineer, Karoly Ereky, coined the word "biotechnology" in 1919, achieving him the title of "Father of Biotechnology."

Principles of Biotechnology:

Among many, the two core techniques that entitled the birth of modern biotechnology are :

  1. Genetic Engineering is one of the methods of direct modifications of an organism's genome (i.e., DNA and RNA). It requires the introduction of new genes into host species to escalate function or trait, hence altering the host organism's phenotype.
  2. Bioprocess Engineering is one of the methods of maintaining sterile conditions to facilitate the growth of large quantities of desired microbes and other eukaryotic cells for the development of new or modified biotechnological products such as enzymes, vaccines, antibiotics, and other biotechnological products.