Step 1: Analyze the function.
\[ f(x) = \frac{x^2 + 1}{x^2 + x + 1} \] The denominator never becomes zero for real $x$.
Step 2: Check monotonicity.
Compute derivative: \[ f'(x) = \frac{(2x)(x^2 + x + 1) - (x^2 + 1)(2x + 1)}{(x^2 + x + 1)^2} = \frac{x(x-1)}{(x^2 + x + 1)^2} \] $f'(x)>0$ for $x>1$ and $f'(x)<0$ for $0<x<1$. Thus, the function is decreasing for $x<1$ and increasing for $x>1$.
Step 3: Identify one-to-one intervals.
The function is one-to-one on $(-\infty, 0]$ and $[1, \infty)$. For natural numbers ($x = 1, 2, 3, ...$), it is strictly increasing → one-to-one.
Step 4: Conclusion.
Hence, $f(x)$ is one-to-one on natural numbers.
Identify the taxa that constitute a paraphyletic group in the given phylogenetic tree.
The vector, shown in the figure, has promoter and RBS sequences in the 300 bp region between the restriction sites for enzymes X and Y. There are no other sites for X and Y in the vector. The promoter is directed towards the Y site. The insert containing only an ORF provides 3 fragments after digestion with both enzymes X and Y. The ORF is cloned in the correct orientation in the vector using the single restriction enzyme Y. The size of the largest fragment of the recombinant plasmid expressing the ORF upon digestion with enzyme X is ........... bp. (answer in integer) 